Methodology
Immunohistochemistry (IHC)
Test Description
Smoothelin is a novel cytoskeletal protein that reacts with the 59 kDa and 100 kDa proteins corresponding to Smoothelin A and B, respectively, which are exclusively found in smooth muscle cells (SMC). Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain Smoothelin. Smoothelin is exclusively expressed in fully differentiated (contractile) smooth muscle cells and could be used as a tool to differentiate muscularis propria from muscularis mucosa.
Specimen Requirements
  • A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type

    or

  • One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered

  • Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.

Storage and Transportation
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88342 x 1 or 88341 x 1
Turnaround Time
24 hours
Level of Service
Stain Only

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.