Multiplexed Immunofluorescence: a complete view of tumor behavior
Utilizing deep learning and advanced cell classification algorithms, multiplexed immunofluorescence spatial analysis uncovers the complex interplay between multitudes of immuno-oncology biomarkers and provides complete analysis of the tumor microenvironment. Multiplexed immunofluorescence has emerged as an effective and proficient approach to simultaneously identify specific proteins and immune cell types, to determine the spatial distribution and activation state of immune cells, as well as the expression of immune modulators, all at the same time. This method is highly beneficial for exploring immune evasion mechanisms and finding potential biomarkers that allow researchers to assess the mechanism of action and predict and monitor drug response.
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NeoGenomics’ Pharma Services offer two multiplex immunofluorescence platforms: MultiOmyx™ and PhenoImager™ HT
MultiOmyx™ is a proprietary, high-order multiplexing methodology that enables visualization and characterization of up to 60 proteins in a single FFPE tissue section.
High order ≥ 7 plex
Comprehensive analysis of tumor biology
Deep & precise immuno-profiling
PhenoImager™ HT (formerly Vectra Polaris) is an automated system that allows simultaneous detection of 2-6 different markers from a single FFPE tissue slide which would provide a streamlined workflow for implementing refined low-order multiplex panel sets.
Low order 2-6 plex
High-throughput & faster TAT
Assay transfer & validation
Latest Publications:
Characterizing CD39 and CD73 cell subtypes in the tumor microenvironment using MultiOmyx immunofluorescence assay
Comprehensive analysis of natural killer cell-associated markers using MultiOmyx immunofluorescence assay
Single-cell immunoprofiling and spatial analysis of hormone receptor subtypes in HER2+ and HER2low breast tumors using multiplexed immunofluorescence
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