Multiplexed Immunofluorescence


Multiplexed Immunofluorescence: a complete view of tumor behavior

Utilizing deep learning and advanced cell classification algorithms, multiplexed immunofluorescence spatial analysis uncovers the complex interplay between multitudes of immuno-oncology biomarkers and provides complete analysis of the tumor microenvironment. Multiplexed immunofluorescence has emerged as an effective and proficient approach to simultaneously identify specific proteins and immune cell types, to determine the spatial distribution and activation state of immune cells, as well as the expression of immune modulators, all at the same time. This method is highly beneficial for exploring immune evasion mechanisms and finding potential biomarkers that allow researchers to assess the mechanism of action and predict and monitor drug response.

NeoGenomics’ Pharma Services offer two multiplex immunofluorescence platforms: MultiOmyx and PhenoImager HT


MultiOmyx is a proprietary, high-order multiplexing methodology that enables visualization and characterization of up to 60 proteins in a single FFPE tissue section.

High order ≥ 7 plex
Comprehensive analysis of tumor biology
Deep & precise immuno-profiling

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Akoya PhenoImager™

PhenoImager HT (formerly Vectra Polaris) is an automated system that allows simultaneous detection of 2-6 different markers from a single FFPE tissue slide which would provide a streamlined workflow for implementing refined low-order multiplex panel sets.

Low order 2-6 plex
High-throughput & faster TAT
Assay transfer & validation

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Latest Publications:

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PAK4 as a potential marker for poor response to immunotherapy in melanoma patients

ESMO 2022
Sep 9, 2022

Spatial interrogation of tertiary lymphoid structures (TLS) in colorectal carcinoma (CRC) tumor microenvironment (TME) using the MultiOmyxTM assay

AACR 2022
Apr 14, 2022

Quantitation of CD137 and Nectin-4 expression across multiple tumor types to support indication selection for BT7480, a Bicycle TICA™

SITC 2021
Nov 13, 2021

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