Alternative Name
11q Gain / Loss
Methodology
FISH
Test Description
Probes: CEN 11 (11p11.1-q11) | MLL (11q23) | 11qTel (11q25)
Disease(s): B-cell non-Hodgkin lymphoma
Clinical Significance
This FISH panel detects proximal gains and distal losses of chromosome 11q which are recurrent abnormalities in MYC-negative high grade B-cell lymphomas resembling Burkitt lymphoma. "Burkitt-like lymphoma with 11q aberration" was recognized by the WHO in 2017 as a new provisional entity. This pattern of 11q abnormalities is also observed in MYC-positive Burkitt lymphomas and MYC-positive high-grade B-cell lymphomas, not otherwise specified.
Specimen Requirements
- Bone Marrow Aspirate: N/A
- Peripheral Blood: N/A
- Fresh, Unfixed Tissue: N/A
- Fluids: N/A
- Paraffin Block: H&E slide (required) plus paraffin block. Circle H&E for tech-only.
- Cut Slides: H&E slide (required) plus 2 unstained slides cut at 4 microns. Circle H&E for tech-only.
Storage & Transportation
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88374x1 automated or 88377x1 manual
Turnaround Time
3-5 days
Level of Service
Global
Technical
New York Approved
Yes
References
- Swerdlow SH, et al. WHO classification of tumors of hematopoietic and lymphoid tissues (Revised 4th edition). IARC Press, Lyon 2017
- Salaverria I, et al. A recurrent 11q aberration pattern characterizes a subset of MYC-negative high-grade B-cell lymphomas resembling Burkitt lymphoma. Blood. 2014;123:1187-1198.
- Ferreiro JF et al. Post-transplant molecularly defined Burkitt lymphomas are frequently MYC-negative and characterized by the 11q-gain/loss pattern. Haematologica. 2015;100:e275-e279.
- Grygalewicz B, et al. The 11q-gain/loss aberration occurs recurrently in MYC-negative Burkitt-like lymphoma with 11q aberration, as well as MYC-positive Burkitt lymphoma and MYC-positive high-grade B-cell lymphoma, NOS. Am J Clin Pathol. 2018;149:17-28.