Alternative Name
Acute myeloid leukemia
Methodology
FISH
Test Description
Probes: RPN1, MECOM (3q21, 3q26.2) | 5q-, -5 (5p15, 5q31, 5q33 | 7q-, -7 (Cen 7, 7q22, 7q31) | Trisomy 8 (Cen 8) | DEK/NUP214 (CAN) t(6;9) | MLL (11q23) | ETV6 (12p13) | 17p- (TP53 17p13.1, NF1 17q11.2) | Probes may be ordered separately.
Disease(s):Acute myeloid leukemia
Clinical Significance
The AML Non-Favorable Risk FISH Panel accommodates US and international cytogenetic risk classifications for intermediate and adverse risk groups. This Panel was formerly called AML Extended Panel.
Specimen Requirements
- Bone marrow aspirate: 1-2 mL sodium heparin tube. EDTA tube is acceptable.
- Peripheral blood: 2-5 mL sodium heparin tube. EDTA tube is acceptable.
- Fresh, unfixed tissue: Tissue in RPMI.
- Bone Marrow/ Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: minimum 8 slides labeled with specimen type.
- Fluids: Equal parts RPMI to specimen volume.
- Fixed Cell Suspension: A client fixed cell suspension may be submitted for testing as long as it is received in 3:1 Methanol:Glacial Acetic Acid.
- Paraffin block or cut slides: Not available.
- Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Storage & Transportation
Refrigerate specimen. Do not freeze. Use cold pack for transport. Make sure cold pack is not in direct contact with specimen. For fresh samples: ship same day as drawn whenever possible; specimens <72 hours old preferred.
CPT Code(s)*
88374x8 automated. Codes may differ if manual analysis is performed.
Turnaround Time
3-5 days
Level of Service
Technical
Global
New York Approved
Yes