Rely on our flow cytometry expertise and capabilities to deliver on your project.
Our scientific and medical teams are experienced in flow cytometry assessments supporting many applications including hematopoietic neoplasms, MRD, receptor occupancy, expansive immunophenotyping, and Immuno-oncology assessments. NeoGenomics also offers other supporting services, such as global PBMC processing for retrospective or functional testing. Harmonized processes and procedures are used across all global labs for continuity and consistency through longitudinal, global clinical trials.
Global, with all sites CAP accredited
Harmonized instrument calibration and specific reagent qualification for consistent assay performance globally
Robust and controlled assay transfers with data reproducibility at all global testing locations
Collaborative and consultative approach
Dedicated pathology review (if required)
Harmonized SOPs
Flow Assay Design
Intended Use
MRD status
Malignant cell characterization
CAR-T detection with custom anti-CAR-T antibody
TBNK enumeration
Immunophenotyping (subsets, MD SC/dendritic, activation/exhaustion, rare event, etc.)
Receptor occupancy
Signal transduction/cell signaling
Intracellular cytokine/cell function
Checkpoint molecules
Apoptosis
Example Assays and Reports
CLL MRD
- 0.01% sensitivity
- 300K-1 million events
- Single tube Duraclone assay (8 color)
| Fluorochrome | FITC | PE | PC 5.5 | PE-Cy7 | APC | APC 750 | PB | KO |
| CLL MRD | CD81 | ROR1 | CD79B | CD19 | CD5 | CD53 | CD20 | CD45 |
B-ALL MRD
- 0.01% sensitivity
- 300K-1 million events
- 3-tube assay
| Fluorochrome | FITC | PE | PC 5.5 | PE-Cy7 | APC | APC 750 |
| B-ALL Tube 1 | CD20 | CD10 | CD38 | CD19 | CD58 | CD45 |
| B-ALL Tube 2 | CD9 | CD13/CD33 | CD34 | CD19 | CD10 | CD45 |
| B-ALL Tube 3 | Syto 16 | - | CD3 | CD19 | CD71 | CD45 |
MM MRD
- 0.001% sensitivity
- 3-5 million events collected
- Single tube assay (10 color)
| Fluorochrome | FITC | PE | PC 5.5 | PE-Cy7 | BV 421 | BV510 | APC | APC- A700 | PB | APC-H7 |
| MM MRD | cKappa | cLambda | CD117 | CD19 | CD81 | CD38 | CD27 | CD138 | CD56 | CD45 |
Example: Custom assay designed for immunophenotyping of T-cells, B-cells and monocytes
| Cell Type | Gating Strategy |
| Leukocytes | CD45+ |
| T cells | CD45+CD3+ |
| CD4+ | CD45+CD3+CD4+ |
| CD8+ | CD45+CD3+CD8+ |
| Naive T-cells | CD45+CD3+CD45RA+ |
| Memory T-cells | CD45+CD3+CD45RO+ |
| Transitional T-cells | CD45+CD3+CD45RA+CD45RO+ |
| B-cells | CD45+CD19+ |
| Monocytes | CD45+CD14+ |
Custom flow cytometry assays can be developed in house or transferred from client and/or partner laboratory.
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