Flow Cytometry Services

Comprehensive and Customizable Offerings

  • Global capabilities
    • Global, with all sites CAP accredited
    • Harmonized instrument calibration and specific reagent qualification for consistent assay performance globally
    • Robust and controlled assay transfers with data reproducibility at all global testing locations
  • Custom assay development and validation
    • Collaborative and consultative approach
    • Dedicated pathology review (if required)
    • Harmonized SOPs

16 Color Flow

BD Machine

3 days TAT

Flow Image

Assays Validated for the Intended Use

  • Primary/secondary endpoint & safety with full pathology review (LDT)
  • Exploratory (fit for purpose)


Flow Assay Design


Flow Assay Optimization, Validation, & Transfer

  • Assay design/optimization (1-3 weeks)
    - Antibody/clone/fluorochrome
    - Sample type: whole blood, bone marrow aspirate, apheresis, PBMC/BMMC, etc.
  • Assay validation (4-6 weeks)
    - Customize assay design & data reporting
    - Client consultation and interaction
    - Access to de-identified patient material/malignancies for validation purposes
    - LDT validation / fit for purpose validation
  • Assay transfer to Rolle, if needed (2-4 weeks)
    - Customize assay design & data reporting
    - Client consultation
Flow Assay Optimization, Validation, & Transfer

Example Assays and Reports:

Examples of LDT assays and reports

CLL MRD

  • 0.01% sensitivity
  • 300K-1 million events
  • Single tube Duraclone assay (8 color)
FluorochromeFITCPEPC 5.5PE-Cy7APCAPC 750PBKO
CLL MRDCD81ROR1CD79BCD19CD5CD53CD20CD45
CLL MRD Image

B-ALL MRD

  • 0.01% sensitivity
  • 300K-1 million events
  • 3-tube assay
Fluorochrome FITCPEPC 5.5PE-Cy7APCAPC 750
B-ALL Tube 1CD20CD10CD38CD19CD58CD45
B-ALL Tube 2CD9CD13/CD33CD34CD19CD10CD45
B-ALL Tube 3Syto 16-CD3CD19CD71CD45
B-ALL MRD Image

MM MRD

  • 0.001% sensitivity
  • 3-5 million events collected
  • Single tube assay (10 color)
FluorochromeFITCPEPC 5.5PE-Cy7BV 421BV510APCAPC- A700PBAPC-H7
MM MRDcKappacLambdaCD117CD19CD81CD38CD27CD138CD56CD45
MM MRD Image

Examples of Exploratory assays and reports

Example: Custom assay designed for immunophenotyping of T-cells, B-cells and monocytes

Exploratory Flow Cytometry Image
Cell TypeGating Strategy
LeukocytesCD45+
T cellsCD45+CD3+
CD4+CD45+CD3+CD4+
CD8+CD45+CD3+CD8+
Naive T-cellsCD45+CD3+CD45RA+
Memory T-cellsCD45+CD3+CD45RO+
Transitional T-cellsCD45+CD3+CD45RA+CD45RO+
B-cellsCD45+CD19+
MonocytesCD45+CD14+

Custom flow cytometry assays can be developed in house or transferred from client and/or partner laboratory.