Flow Cytometry

Rely on our flow cytometry expertise and capabilities to deliver on your project.

Our scientific and medical teams are experienced in flow cytometry assessments supporting many applications including hematopoietic neoplasms, MRD, receptor occupancy, expansive immunophenotyping, and Immuno-oncology assessments. NeoGenomics also offers other supporting services, such as global PBMC processing for retrospective or functional testing.  Harmonized processes and procedures are used across all global labs for continuity and consistency through longitudinal, global clinical trials.

Flow Cytometry
Comprehensive and Customizable Offerings
Global reach
Global Capabilities

Global, with all sites CAP accredited
Harmonized instrument calibration and specific reagent qualification for consistent assay performance globally
Robust and controlled assay transfers with data reproducibility at all global testing locations

Regulated Seq Pharma icon
Custom assay development and validation

Collaborative and consultative approach
Dedicated pathology review (if required)
Harmonized SOPs

Flow Assay Design

Intended Use

Primary/secondary endpoint & safety with full pathology review (LDT)

MRD status
Malignant cell characterization
CAR-T detection with custom anti-CAR-T antibody
TBNK enumeration

Exploratory (fit for purpose)

Immunophenotyping (subsets, MD SC/dendritic, activation/exhaustion, rare event, etc.)
Receptor occupancy
Signal transduction/cell signaling
Intracellular cytokine/cell function
Checkpoint molecules
Apoptosis

Example Assays and Reports

Examples of LDT assays and reports

CLL MRD

  • 0.01% sensitivity
  • 300K-1 million events
  • Single tube Duraclone assay (8 color)
Fluorochrome FITC PE PC 5.5 PE-Cy7 APC APC 750 PB KO
CLL MRD CD81 ROR1 CD79B CD19 CD5 CD53 CD20 CD45

 

CLL MRD

B-ALL MRD

  • 0.01% sensitivity
  • 300K-1 million events
  • 3-tube assay
Fluorochrome  FITC PE PC 5.5 PE-Cy7 APC APC 750
B-ALL Tube 1 CD20 CD10 CD38 CD19 CD58 CD45
B-ALL Tube 2 CD9 CD13/CD33 CD34 CD19 CD10 CD45
B-ALL Tube 3 Syto 16 - CD3 CD19 CD71 CD45

 

B ALL MRD

MM MRD

  • 0.001% sensitivity
  • 3-5 million events collected
  • Single tube assay (10 color)
Fluorochrome FITC PE PC 5.5 PE-Cy7 BV 421 BV510 APC APC- A700 PB APC-H7
MM MRD cKappa cLambda CD117 CD19 CD81 CD38 CD27 CD138 CD56 CD45

 

MM MRD
Examples of Exploratory assays and reports

Example: Custom assay designed for immunophenotyping of T-cells, B-cells and monocytes

 

Exploratory Flow Cytometry
Cell Type Gating Strategy
Leukocytes CD45+
T cells CD45+CD3+
CD4+ CD45+CD3+CD4+
CD8+ CD45+CD3+CD8+
Naive T-cells CD45+CD3+CD45RA+
Memory T-cells CD45+CD3+CD45RO+
Transitional T-cells CD45+CD3+CD45RA+CD45RO+
B-cells CD45+CD19+
Monocytes CD45+CD14+

Custom flow cytometry assays can be developed in house or transferred from client and/or partner laboratory.

Contact Us To Get Started!

Work with us – your premier oncology partner – to optimize cancer care for patients