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Alternative Name
Acid Fast Bacillus
Immunohistochemistry (IHC)
Test Description

Special stain. Ziehl-Neelsen Acid-Fast Bacilli Stain is used to detect the presence of acid-fast mycobacteria in tissue sections. Acid-fast techniques are of value in the detection of mycobacteria, rod-shaped organisms that sometimes exhibit filamentous (fungus-like) growth. The most significant disease-producing mycobacteria are Mycobacterium tuberculosis and Mycobacterium leprae.

Specimen Requirements
  • A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
  • One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
  • Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage and Transportation

Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.

CPT Code(s)*
New York Approved
Level of Service
Stain Only
Turnaround Time

Global: 48 hours, Tech-Only (stain only): 24 hours

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.