CDX2 is a transcription factor expressed in the nuclei of epithelial cells throughout the intestine, from duodenum to rectum. The CDX2 protein is expressed in primary and metastatic colorectal carcinomas and has also been demonstrated in the intestinal metaplasia of the stomach and intestinal-type gastric cancer. It is not expressed in the normal gastric mucosa. Cytokeratin 7 is a basic cytokeratin and is expressed in epithelial cells of ovary, lung, and breast, but not of the colon or gastrointestinal tract. This antibody cocktail of CDX2 and CK7 can be used simultaneously to distinguish stomach and colon cancers from breast, lung, and ovarian cancers.
- A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
- One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
- Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.