Bi-directional sequencing of PIK3CA exons 1, 9, and 20 which are the most commonly-mutated regions of the gene.
The PIK3CA gene encodes the p110 alpha catalytic subunit of PI3K enzymes. Mutations occur in a wide variety of tumors and may have prognostic and therapeutic significance, depending on tumor type. Numerous PI3K-pathway inhibitors are in development.
- FFPE solid tumor tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.