Methodology
FISH
Test Description
Probes: CBFB inv(16), t(16;16)
Disease(s): AML, AMML (AML-M4E)
Clinical Significance
Available separately or as part of the AML FISH Panel.
Specimen Requirements
- Bone Marrow Aspirate: 1-2mL Sodium Heparin Tube. EDTA tube is acceptable
- Peripheral Blood: 2-5mL Sodium Heparin Tube. EDTA tube is acceptable
- Fresh, Unfixed Tissue: Tissue in RPMI
- Bone Marrow/ Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: minimum 1 slide labeled with specimen type.
- Fluids: Equal parts RPMI to specimen volume
- Fixed Cell Suspension: A client fixed cell suspension may be submitted for testing as long as it is received in 3:1 Methanol:Glacial Acetic Acid.
- Paraffin or Cut Slides: N/A
- Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Storage & Transportation
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: ship same day as drawn whenever possible; specimens <72 hours old preferred.
CPT Code(s)*
88374x1 automated. Codes may differ if manual analysis is performed.
Turnaround Time
3-5 days
Level of Service
Technical
Global
New York Approved
Yes