Probes: FGFR3/IgH t(4;14) | CCND1/IgH t(11;14) | IgH/MAF t(14;16) | IgH/MAFB t(14;20)
Probes for each translocation may be ordered separately.
This panel is available separately or by reflex after the Plasma Cell Myeloma FISH Panel if it detects IgH rearrangement.
Disease(s): Plasma cell myeloma, multiple myeloma
Note: Plasma cell enrichment will be performed on bone marrow samples unless our client directs us otherwise. (Peripheral blood is not recommended as a screening specimen unless increased plasma cells are seen on blood smear.) Specimens should be received in our laboratory within 72 hours of collection.
The panel is performed when IgH is rearranged. This panel is used to identify the IgH partner gene in myeloma, which has prognostic impact. IgH rearrangements are found in 55-70% of myelomas. Together t(4;14), t(11;14), t(14;16) and t(14;20) are found in approximately 35% of myelomas and in 90% of myelomas when there is an IgH gene rearrangement present.
To learn more about the importance of FISH testing with plasma cell enrichment in multiple myeloma, please visit Multiple Myeloma Cytogenetic Analysis resource page.
- Bone Marrow Aspirate: 1-2mL Sodium Heparin Tube. EDTA tube is acceptable
- Peripheral Blood: Plasma cell enrichment was not validated using peripheral blood; therefore, it is not recommended as a screening specimen unless increased plasma cells are seen on blood smear. 2-5mL sodium heparin tube. EDTA tube is acceptable.
- Fresh, Unfixed Tissue: Tissue in RPMI
- Bone Marrow/ Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: minimum 4 slides labeled with specimen type.
- Fluids: Equal parts RPMI to specimen volume.
- Fixed Cell Suspension: A client fixed cell suspension may be submitted for testing as long as it is received in 3:1 Methanol:Glacial Acetic Acid.
- Paraffin Block or Cut Slide: N/A
- Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. Specimens should be received in our laboratory within 72 hours of collection.
3-5 days