Methodology
Immunohistochemistry (IHC)
Test Description
Phosphatase and tensin homolog (PTEN) is a tumor suppressor gene that is mutated in a wide range of cancers. PTEN plays a role in cell proliferation, apoptosis and migration. Reduced expression of PTEN has been reported in a variety of malignancies, including breast, prostate and endometrial cancer, and may be observed in Cowden syndrome tumors. In breast and prostate cancer, loss of PTEN expression has been shown to correlate positively with advanced stage disease. Recent studies have reported that PTEN may be a powerful predictor of response to Herceptin in HER2 positive breast cancer.
Specimen Requirements
- A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
or - One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
- Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage & Transportation
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88342 x 1 or 88341 x 1; 88360 x 1
Turnaround Time
Global: 48 hours, Tech-Only (stain only): 24 hours
Level of Service
Global
Stain Only
New York Approved
Yes