Methodology
Immunohistochemistry (IHC)
Test Description
Perforin is a protein found in cytoplasmic granules of cytotoxic T-lymphocytes (CTLs). CTLs bind to cells that express foreign antigens and induce them to lyse. Perforin expression is significantly induced in CD8 positive T-cells, but to lesser extent in gamma/delta T-cells and NK cells. This antibody may be of value in the detection of perforin in CTLs in severe cases of graft versus host disease, chronic renal rejection and peripheral T-cell lymphomas. In addition, perforin antibody may also be useful for the detection of NK cell lymphomas, all of which express the perforin protein.
Specimen Requirements
- A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
or - One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
- Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage & Transportation
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88342 x 1 or 88341 x 1
Turnaround Time
24 hours
Level of Service
Stain Only
New York Approved
Yes