The NeoTYPE Liposarcoma Fusion Profile combines next-generation sequencing to detect translocations in the genes EWSR1, FUS, HMGA2, and PLAG1 with FISH testing for MDM2 to detect amplifications relevant in liposarcoma. FISH components of NeoTYPE Profiles may be ordered as “Tech-Only” by pathology clients who wish to perform the professional component.
The diagnosis and classification of lipomatous lesions can be very challenging. Fortunately, reproducible cytogenetics have been identified in the most common lipomatous neoplasms. MDM2 amplification is a very consistent feature of well-differentiated liposarcoma/atypical lipomatous tumor and their dedifferentiated counterparts, while other abnormalities have been identified in lipoblastoma, lipoma, and myxoid/round cell liposarcoma. This panel, combining MDM2 FISH with selected NGS studies, has been designed to help in the diagnosis of the major lipopomatous neoplasms and provide clear distinction from one another.
FFPE tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Use cold pack for transporting block during summer to prevent block from melting. Slides can be packed at room temperature.