Methodology
Immunohistochemistry (IHC)
Test Description
Nuclear expression of myogenic differentiation 1 (MyoD1) is restricted to skeletal muscle tissue and has been demonstrated to be a sensitive marker of myogenic differentiation. The antibody strongly labels the nuclei of myoblasts in developing skeletal muscle tissue, whereas the majority of adult skeletal muscle is negative. MyoD1 immunostaining has been demonstrated in the majority of rhabdomyosarcomas of various histological subtypes.
Specimen Requirements
- A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
or - One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
- Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage & Transportation
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88342 x 1 or 88341 x 1
Turnaround Time
24 hours
Level of Service
Stain Only
New York Approved
Yes