Methodology
Immunohistochemistry (IHC)
Test Description
Mismatch repair (MMR) genes results in failure to repair errors in repetitive sequences that occur during DNA replication. The defects in DNA repair pathways have been related to tumor carcinogenesis. MSH6 mutations appear to be associated with atypical HNPCC and in particular with development of endometrial carcinoma or atypical endometrial hyperplasia, the presumed precursor of endometrial cancer. Defects in MSH6 are also found in familial colorectal cancers (suspected or incomplete HNPCC).
Specimen Requirements
- A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
or - One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
- Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage & Transportation
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88342 x 1 or 88341 x 1; 88360 x 1; 88361 x 1
Turnaround Time
Global: 48 hours, Image Analysis (tech-only): 48 hours, Tech-Only (stain only): 24 hours
Level of Service
Stain Only
Global
New York Approved
Yes