Bi-directional sequencing of exons 2, 3, and 4 of the KRAS (includes G12C mutation) gene including codons 12, 13, 59, 61, 117, and 146. High-sensitivity sequencing is used for enhanced detection of mutations in codons 12 and 13. Testing is available separately or in combination with BRAF, HRAS and NRAS in the RAS/RAF Panel.
Testing is recommended in colorectal cancer as mutations are associated with resistance and shorter overall survival with EGFR-antagonist therapies such as cetuximab or panitumumab. Testing in non-small cell lung cancer may provide prognostic information and predict poor response to EGFR tyrosine kinase inhibitors.
- FFPE solid tumor tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
- Fine needle aspirate (FNA): FFPE cell blocks are acceptable. Requisition must note specimen is FNA. Fresh cells and smears are not acceptable.
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.