Akoya PhenoImager™: High Throughput Multiplexing Methodology
NeoGenomics offers an end-to-end PhenoImager™ service solution with our Akoya Biosciences partnership, using an optimized and high-throughput workflow beginning with custom assay development through multiplex imaging and analysis of 2-6 markers on a single FFPE slide. PhenoImager™ HT (formerly Vectra Polaris) is an integrated, fully-enclosed instrument with touchless slide automation that allows for a streamlined staining, imaging and analysis workflow ideal for implementing optimized, low-order multiplex panel sets with the potential for real-time testing applications.
PhenoImager multiplexing services by NeoGenomics include:
- Automated staining workflow with Opal TSA-based amplification kit
- Whole slide multispectral imaging at high throughput with up to 6 markers with continuous slide loading (80+ slides)
- Expert medical, scientific, and bioinformatics consultation within a single organization
- Custom assay design and verification
- Assay transfer and validation
- Unambiguous immune cell co-expression and co-localization
- Spatial analytics
- Integrated with RNAScope®
PhenoImager Image Analysis:
- Phenochart & inForm®: spectrally unmixing images, AFR and pathology views
- HALO® or Visiopharm®: tissue segmentation, cell segmentation, phenotyping, scoring and spatial distribution analysis
For more details on image analysis workflows and capabilities offered by NeoGenomics go to:
https://neogenomics.com/pharma-services/lab-services/multiplexing-if/data-image-analysis
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