Bi-directional sequencing of TP53 exons 4-9.
TP53 mutations are detected in at least 50% of all adult tumors and are generally associated with a poor prognosis. The TP53 gene encodes the tumor suppressor p53. Germline mutations in TP53 are the cause of Li-Fraumeni Syndrome.
Peripheral blood: 5 mL in EDTA tube. Bone marrow: 2 mL in EDTA tube. FFPE solid tumor tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.