Methodology
Immunohistochemistry (IHC)
Test Description
PRAME (PReferentially expressed Antigen in MElanoma) is expressed in about 90% among melanoma subtypes, while negative in about 85% of cutaneous melanocytic nevi. Immunohistochemical analysis of PRAME may be useful for diagnostic purposes to support diagnosis of melanoma.
Clone: EPR20330
Staining pattern: Nuclear
Specimen Requirements
- A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
or - One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
- Note: Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage & Transportation
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88342x1 or 88341x1
Turnaround Time
24 Hours
Level of Service
Stain Only
New York Approved
Yes
References
- Lezcano, C. et al. PRAME Expression in Melanocytic Tumors. Am J Surg Pathol. 2018 Nov;42(11):1456-1465.