PRAME (PReferentially expressed Antigen in MElanoma) is expressed in about 90% among melanoma subtypes, while negative in about 85% of cutaneous melanocytic nevi. Immunohistochemical analysis of PRAME may be useful for diagnostic purposes to support diagnosis of melanoma.
Staining pattern: Nuclear
- A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
- One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
- Note: Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
- Lezcano, C. et al. PRAME Expression in Melanocytic Tumors. Am J Surg Pathol. 2018 Nov;42(11):1456-1465.