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Immunohistochemistry (IHC)
Test Description

PRAME (PReferentially expressed Antigen in MElanoma) is expressed in about 90% among melanoma subtypes, while negative in about 85% of cutaneous melanocytic nevi. Immunohistochemical analysis of PRAME may be useful for diagnostic purposes to support diagnosis of melanoma.

Clone: EPR20330
Staining pattern: Nuclear

Specimen Requirements
  • A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
  • One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
  • Note: Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage and Transportation

Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.

CPT Code(s)*
88342x1 or 88341x1
New York Approved
Level of Service
Stain Only
Turnaround Time

24 Hours

  1. Lezcano, C. et al. PRAME Expression in Melanocytic Tumors. Am J Surg Pathol. 2018 Nov;42(11):1456-1465.

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.