Methodology
FISH
Test Description
Probes: PML/RARA t(15;17)
Disease(s): AML, APL (AML-M3)
Note: PML-RARA FISH is performed STAT when ordered as a stand-alone test (outside a panel). Note MD contact name and phone number to receive STAT results.
Clinical Significance
Available separately or as part of the AML Standard FISH Panel and AML Favorable-Risk Panel.
Specimen Requirements
- Bone Marrow Aspirate: 1-2mL Sodium Heparin Tube. EDTA tube is acceptable
- Peripheral Blood: 2-5mL Sodium Heparin Tube. EDTA tube is acceptable
- Fresh, Unfixed Tissue: Tissue in RPMI
- Bone Marrow/ Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: minimum 1 slide labeled with specimen type.
- Fluids: Equal parts RPMI to specimen volume.
- Fixed Cell Suspension: A client fixed cell suspension may be submitted for testing as long as it is received in 3:1 Methanol:Glacial Acetic Acid.
- Paraffin Block or Cut Slides: N/A
- Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Storage & Transportation
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: ship same day as drawn whenever possible; specimens <72 hours old preferred.
CPT Code(s)*
88374x1 automated. Codes may differ if manual analysis is performed.
Turnaround Time
4 days. STAT results are reported 12-24 hours from receipt in the NeoGenomics laboratory.
Level of Service
Technical
Global
New York Approved
Yes