Probes: 1p-, 1q+, iso(1q): CDKN2C (1p32), CKS1B (1q21) | +5, hyperdiploidy (5p15) | +9, hyperdiploidy (9q22) | +15, hyperdiploidy (15q22) | 13q- (13q14, 13q34) | IgH (14q32) | 17p- (TP53 17p13.1, NF1 17q11.2)
Probes may be ordered separately except +5, +9 and +15 which are combined.
Global cases with IgH rearrangement will automatically reflex to the Plasma Cell Myeloma IgH Complex FISH Panel unless client has opted out.
Disease(s): Plasma cell myeloma, multiple myeloma
Note: Plasma cell enrichment will be performed on bone marrow samples unless our client directs us otherwise. (Peripheral blood is not recommended as a screening specimen unless increased plasma cells are seen on blood smear.) Specimens should be received in our laboratory within 72 hours of collection.
The panel is used for the detection of FISH and chromosome aberrations useful in prognosis in plasma cell myeloma. As malignant plasma cells often have a low proliferation index, conventional cytogenetics frequently yields normal results. When this happens, interphase FISH studies can increase the abnormality detection rate.
- Bone Marrow Aspirate: 1-2mL Sodium Heparin Tube. EDTA tube is acceptable
- Peripheral Blood: Plasma cell enrichment was not validated using peripheral blood; therefore, it is not recommended as a screening specimen unless increased plasma cells are seen on blood smear. 2-5mL Sodium Heparin Tube. EDTA tube is acceptable
- Fresh, Unfixed Tissue: Tissue in RPMI
- Bone Marrow/ Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: minimum 5 slides labeled with specimen type.
- Fluids: Equal parts RPMI to specimen volume.
- Fixed Cell Suspension: A client fixed cell suspension may be submitted for testing as long as it is received in 3:1 Methanol:Glacial Acetic Acid.
- Paraffin Block or Cut Slide: N/A
- Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.Specimens should be received in our laboratory within 72 hours of collection.
3-5 days. Add 3-5 days if reflexed.