Alternative Name
NHL FISH
Methodology
FISH
Test Description
Probes: ALK (2p23) | BCL6 (3q27) | MYC (8q24) | CCND1/IgH t(11;14) | IgH (14q32) | IgH/BCL2 t(14;18) | MALT1 (18q21)
Probes may be ordered separately.
Disease(s): NHL
Clinical Significance
The NHL FISH panel is used for the detection of chromosome aberrations observed in lymphoma.
Specimen Requirements
- Bone Marrow Aspirate: 1-2mL Sodium Heparin Tube. EDTA tube is acceptable
- Peripheral Blood: 2-5mL Sodium Heparin Tube. EDTA tube is acceptable
- Fresh, Unfixed Tissue: Tissue in RPMI
- Bone Marrow/ Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: minimum 7 slides labeled with specimen type.
- Fluids: Equal parts RPMI to specimen volume.
- Fixed Cell Suspension: A client fixed cell suspension may be submitted for testing as long as it is received in 3:1 Methanol:Glacial Acetic Acid.
- Paraffin Block: H&E slide (required) plus paraffin block. Circle H&E for tech-only.
- Cut Slide: H&E slide (required) plus 12 unstained slides cut at 4 microns for panel, or 2 unstained slides cut at 4 microns for single abnormalities. Circle H&E for tech-only.
- Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Storage & Transportation
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: ship same day as drawn whenever possible; specimens <72 hours old preferred.
CPT Code(s)*
88374x7 automated. Codes may differ if manual analysis is performed.
Turnaround Time
3-5 days
Level of Service
Technical
Global
New York Approved
Yes