This test is performed by multiple methods to detect mutations in the following genes BCL1, BCL2, BCL6, BRAF, CARD11, CD79B, EZH2, MYD88, NOTCH1, NOTCH2, NRAS and TP53. The test is performed by sequencing the entire coding regions of the genes listed unless otherwise noted. BCL1/ IgH translocation t(11;14) is performed by real-time PCR and BCL2 t(14;18) is performed by fragment length analysis. Test orders include summary interpretation of all results together.
Genes analyzed in this profile have known roles in lymphoma pathogenesis. Testing is useful for diagnosis, classification, prognosis, and treatment decisions. It is also useful to distinguish between activated B-cell-like (ABC) and germinal center (GC) subtypes, especially in diffuse large B-cell lymphoma (DLBCL).
- Bone marrow (Preferred): 2 mL in EDTA tube.
- Peripheral blood: 5 mL in EDTA tube.
- FFPE tissue: Paraffin block. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative is the recommended fixative. Do not use zinc or mercury fixatives (B5). Highly acidic or prolonged decalcification processes will not yield sufficient nucleic acid to accurately perform molecular studies.
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.