The NeoTYPE® Colorectal Tumor Profile characterizes primary or metastatic colorectal tumors of any histological subtype for the most significant genetic changes relevant to therapy decisions, prognosis, and clinical research. This profile analyzes 44 biomarkers using a combination of next‑generation sequencing (NGS), other molecular methods, FISH, and IHC. Test reports include summary interpretation of all results to help guide treatment decisions.
NGS (36 genes + MSI and TMB)
Reflex Rule: Microsatellite Instability (MSI) NGS result of "indeterminate" will create a reflex to MSI by PCR as long as the tumor percentage is ≥20%.
FISH (3 biomarkers)
MET, PTEN, RET
(tech‑only available)
IHC (3 biomarkers)
* HER2 (Other) w/Gastric Scoring (tech‑only available)
Reflex rule: If global HER2 (Other) IHC with Gastric Scoring results in a score of 2+, the case will reflex to global HER2 (Other) FISH with Gastric Scoring, unless a reflex to tech‑only FISH or a reflex opt‑out is requested.
* PD‑L1 LDT (tech‑only available)
* Pan‑TRK
Reflex rule: If Pan‑TRK IHC is expressed or equivocal, reflex testing to either the NTRK NGS Fusion Panel (default) or the NTRK 1–3 FISH Panel will be added.
NeoTYPE® Colorectal Tumor Profile
- Next Generation Sequencing (NGS)
Specimen Requirements
FFPE tissue: Paraffin block preferred. Please use 10% buffered formalin fixative. Do not use zinc fixatives.
Storage and Transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. NYS clients please provide date and time of Collection.
CPT Code(s)*
81457x1, 88377x3, 88360x2, 88342x1; add 88374x1 if HER2 IHC is reflexed to FISH; add 81479x1 if reflexed to NTRK NGS Fusion Panel (default) or 88374x3 automated (88377x3 manual) if reflexed to NTRK 1-3 FISH Panel
Turnaround time
14 Days
NGS: 14 Days
FISH: 7 Days
IHC: Global: 2 Days
Tech-Only (stain
only): 24 Hours
Potential Reflex Testing (Additional Time):
MSI by PCR: 5 Days
HER2 FISH: 5 Days
NTRK 1-3 FISH: 5 Days
NTRK NGS: 1-3 Days
Level of Service
- Global
Integrations
Request electronic integrations →
New York Approved: Yes
*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.
Biomarkers
DNA Sequencing
| SNVs + Indels | |||||
|---|---|---|---|---|---|
| AKT1 | APC | ARID1A | ATM | BRAF | EGFR |
| EPCAM | ERBB2 | ERBB4 | FBXW7 | FGFR1 | FGFR2 |
| FGFR3 | HRAS | KIT | KRAS | MET | MLH1 |
| MSH2 | MSH6 | MUTYH | NOTCH1 | NRAS | PDGFRA |
| PIK3CA | PMS2 | POLD1 | POLE | PTEN | RNF43 |
| SMAD4 | SMO | SRC | STK11 | TERT | TP53 |
| Genomic Signatures | |||||
|---|---|---|---|---|---|
| MSI | TMB | ||||
Immunohistochemistry (IHC)
| Expression | |||||
|---|---|---|---|---|---|
| HER2 | NTRK1 | NTRK2 | NTRK3 | PD-L1 | |
Fluorescence In Situ Hybridization (FISH)
| Amplification(s) | |||||
|---|---|---|---|---|---|
| HER2 | MET | ||||
| Deletion(s) | |||||
|---|---|---|---|---|---|
| PTEN | |||||
| Rearrangement(s) | |||||
|---|---|---|---|---|---|
| RET | |||||
Last Updated: April 03, 2026