Alternative Name
Myelodysplastic syndrome
Methodology
FISH
Test Description
Probes: 5q-, -5 (5p15, 5q31, 5q33) | 7q-, -7 (Cen 7, 7q22, 7q31) | Trisomy 8 (Cen 8) | MLL (11q23) | 20q- (20q12, 20qter)
Probes may be ordered separately except +8 and 20q- which are combined.
Disease(s): Myelodysplastic syndrome
Clinical Significance
The MDS Standard FISH Panel identifies the most frequent cytogenetic abnormalities associated with favorable, intermediate, and poor risk according to IPSS guidelines (since revised). See also the separate listing for MDS Extended FISH Panel which accommodates the 2012 IPSS-Revised classification.
Specimen Requirements
- Bone Marrow Aspirate: 1-2 mL sodium heparin tube. EDTA tube is acceptable.
- Peripheral Blood: 2-5 mL sodium heparin tube. EDTA tube is acceptable.
- Fresh, Unfixed Tissue: Tissue in RPMI.
- Bone Marrow/ Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: minimum 4 slides labeled with specimen type.
- Fluids: Equal parts RPMI to specimen volume.
- Fixed Cell Suspension: A client fixed cell suspension may be submitted for testing as long as it is received in 3:1 Methanol:Glacial Acetic Acid.
- Paraffin Block or Cut Slides: Not available.
- Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Storage & Transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: ship same day as drawn whenever possible; specimens <72 hours old preferred.
CPT Code(s)*
88374x4 automated. Codes may differ if manual analysis is performed.
Turnaround Time
3-5 days
Level of Service
Technical
Global
New York Approved
Yes