Special stain. Amyloidosis is a rare disease characterized by the deposition of insoluble misfolded proteins in various tissues and organs. Congo red stain is the gold standard for the demonstration of amyloid in tissue sections. The amyloid fibril Congo red complex demonstrates “apple green” birefringence using polarized light microscopy. For cases with positive Congo red staining, immunohistochemical stains for amyloid A, amyloid P and immunoglobulin light chains are available for further evaluation of the amyloid subtype, if clinically indicated.
- A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
- One (1) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and two to three (2-3) positively charged unstained slides cut at 10 microns
- Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
NOTE: Clients should perform manual evaluation of the glass slide with polarized light for complete evaluation. Scanning at NeoGenomics is done with routine light microscopy.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.