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BRAF Mutation Analysis by PCR
- Molecular
BRAF Mutation Analysis Assay is based on PCR amplification and detection of target DNA using complementary primer pairs and oligonucleotide probes labeled with fluorsescent dyes. This assay is designed to detect V600 mutations E, K, D, R, and G. For solid tumors, tumor enrichment is performed before extraction. Expanded coverage for BRAF exons 11 & 15 is available in the RAS/RAF Panel. Testing is available separately or in combination with HRAS, KRAS, and NRAS in the RAS/RAF Panel.
Turnaround time
7 Days
Level of Service
- Global
New York Approved: Yes
Specimen Requirements
FFPE solid tumor tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives. Peripheral blood: 5 mL in EDTA tube. Bone marrow: 2 mL in EDTA tube. Note: Test is DNA-based. Please select Extract & Hold - DNA if specimen hold service is desired.
Storage and Transportation
Please select extract & hold - DNA if specimen hold service is desired. Storage & Transportation: Use cold pack for transport, making sure cold pack is not in direct contact with specimen. NYS clients please provide date and time of Collection.
CPT Code(s)*
81210
*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.
Last Updated: January 22, 2025