Alternative Name
BCL6/MYC Translocation
Methodology
FISH
Test Description
- Probes: BCL6/MYC t(3;8)
- Disease(s): B-cell lymphoma, double-hit lymphoma, triple-hit lymphoma
Clinical Significance
BCL6/MYC translocation is observed recurrently in high-grade B-cell lymphoma, reported in the literature as “pseudo” double-hit or non-Ig/MYC translocation, which has a better prognosis than double hit/triple hit lymphomas with Ig/MYC translocations.
Specimen Requirements
- Bone Marrow Aspirate: 1-2mL Sodium Heparin Tube. EDTA tube is acceptable
- Peripheral Blood: 2-5mL Sodium Heparin Tube. EDTA tube is acceptable
- Fresh, Unfixed Tissue: Tissue in RPMI
- Bone Marrow/Peripheral Blood Smear or Fresh Tissue Preparation Slides: minimum 1 slide labeled with specimen type.
- Fluids: Equal parts RPMI to specimen volume
- Paraffin Block: H&E slide (required) plus paraffin block. Circle H&E for tech-only.
- Cut Slides: H&E slide (required) plus 2 unstained slides cut at 4 microns. Circle H&E for tech only.
Storage & Transportation
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88374x1 automated or 88377x1 manual
Turnaround Time
3-5 days
Level of Service
Global
Technical
New York Approved
Yes