Probes: BCL2 (18q21)
Disease(s): B-cell lymphoma, non-Hodgkin lympoma (NHL), follicular lymphoma (FL), diffuse large B-cell lymphoma (DLBCL)
This BCL2 break-apart probe enables detection of BCL2 rearrangements at 18q21 irrespective of the partner gene. Detection of BCL2 rearrangement aids in diagnosis and classification of follicular lymphoma (FL), diffuse large B-cell lymphoma (DLBCL), and other aggressive B-cell lymphomas in conjunction with clinical, morphologic, and flow cytometric data. The most frequent rearrangement partner is IgH (14q32). Rarely, variant translocations involve the light chain genes Ig lambda (22q11) or Ig kappa (2p12), AFF3 (2q11), or the mu switch region. BCL2 functions in anti-apoptosis and its overexpression is implicated in oncogenesis.
- Bone Marrow Aspirate: 1-2mL Sodium Heparin Tube. EDTA tube is acceptable
- Peripheral Blood: 2-5mL Sodium Heparin Tube. EDTA tube is acceptable
- Fresh, Unfixed Tissue: Tissue in RPMI
- Bone Marrow/ Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: minimum 1 slide labeled with specimen type.
- Fluids: Equal parts RPMI to specimen volume
- Fixed Cell Suspension: A client fixed cell suspension may be submitted for testing as long as it is received in 3:1 Methanol:Glacial Acetic Acid.
- Paraffin Block: H&E slide (required) plus paraffin block. Circle H&E for tech-only
- Cut Slides: H&E slide (required) plus 2 unstained slides cut at 4 microns. Circle H&E for tech-only.
- Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: ship same day as drawn whenever possible; specimens <72 hours old preferred.
3-5 days (for both unfixed and FFPE specimens)