Akoya PhenoImager™

High Throughput Multiplexing Methodology

Akoya PhenoImager™: High Throughput Multiplexing Methodology

NeoGenomics offers an end-to-end PhenoImager service solution with our Akoya Biosciences partnership, using an optimized and high-throughput workflow beginning with custom assay development through multiplex imaging and analysis of 2-6 markers on a single FFPE slide. PhenoImager HT (formerly Vectra Polaris) is an integrated, fully-enclosed instrument with touchless slide automation that allows for a streamlined staining, imaging and analysis workflow ideal for implementing optimized, low-order multiplex panel sets with the potential for real-time testing applications.

Vectra Polaris

PhenoImager multiplexing services by NeoGenomics include:

  • Automated staining workflow with Opal TSA-based amplification kit 
  • Whole slide multispectral imaging at high throughput with up to 6 markers with continuous slide loading (80+ slides)
  • Expert medical, scientific, and bioinformatics consultation within a single organization
  • Custom assay design and verification
  • Assay transfer and validation 
  • Unambiguous immune cell co-expression and co-localization
  • Spatial analytics 
  • Integrated with RNAScope®

PhenoImager Image Analysis:

  • Phenochart & inForm®: spectrally unmixing images, AFR and pathology views
  • HALO® or Visiopharm®: tissue segmentation, cell segmentation, phenotyping, scoring and spatial distribution analysis
     

For more details on image analysis workflows and capabilities offered by NeoGenomics go to:
https://neogenomics.com/pharma-services/lab-services/multiplexing-if/data-image-analysis

Verified Antibody Panels or Custom Panels
We have commonly used antibody panels pre-verified and ready for use immediately (see list below). We can also develop custom panel(s) specific for your study.
Immune Cell Panel #1

CD3
CD4
CD8
FoxP3
CD68
PanCK

Immune Cell Panel #2

CD3
CD163
HLA-DR
CD68
CD56
CD20

Immune Cell Panel #3

CD8
FoxP3
PD-1
CD68
PD-L1
PanCK/SOX10

Immune Cell Panel #4

CD19
CD68
CD56
HLA-DR
PD-L1
PanCK

T cell Panel #1

CD3
CD4
CD8
CD45RO
FoxP3
PanCK

T cell Panel #2

CD4
CD8
PD-1
LAG3
TIGIT

T cell Panel #3

CD3
CD4
CD8
FoxP3
PD-1
PanCK

T cell Panel #4

CD3
CD4
CD8
FoxP3
PanCK

Contact Us To Get Started!

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