Methodology
Flow Cytometry
Test Description

Available as global and tech-only. Markers are CD3, CD5, CD19, CD45, and ZAP-70.

Clinical Significance

Over-expression of ZAP-70 is a poor prognostic marker in CLL/SLL.

Specimen Requirements
  • Bone marrow aspirate or peripheral blood: 1-2 mL EDTA preferred. 1-2 mL minimum in sodium heparin tube or ACD (pale yellow/no gel separator) is acceptable. Please provide recent CBC report.
  • Bone marrow core: 1-2 cm minimum core length in RPMI.
  • Fresh, unfixed tissue: 0.2 cm3 minimum in RPMI.
  • Fluids and FNAs: Equal parts RPMI and specimen.
Storage and Transportation

Specimens must be received at NeoGenomics within 48 hours of collection. Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

CPT Code(s)*
88184, 88185(x4). Add 88187(x1) for global.
Turnaround Time

1 day

Level of Service
Global
Technical

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.