Methodology
Flow Cytometry
Test Description

Available as global test only. Markers are VB1, VB2, VB3, VB4, VB5.1, VB5.2, VB5.3, VB7.1, VB7.2, VB8, VB9, VB11, VB12, VB13.1, VB13.2, VB13.6, VB14, VB16, VB17, VB18, VB20, VB21.3, VB22, and VB23 (24 markers). Two additional T-cell markers are used to identify the population of interest and the markers vary from case to case.

Clinical Significance

Useful in identification of clonal T-cell populations to aid in diagnosis of T-cell lymphoproliferative disorders. Clonal T-cell populations can be identified in some reactive conditions and in some healthy elderly patients. Correlation with morphology, clinical history, and other diagnostic information is necessary for diagnosis.

Specimen Requirements
  • Bone marrow aspirate or peripheral blood: 1-2 mL EDTA preferred. 1-2 mL minimum in sodium heparin tube or ACD (pale yellow/no gel separator) is acceptable. Please provide recent CBC report.
  • Bone marrow core: 1-2 cm minimum core length in RPMI.
  • Fresh, unfixed tissue: 0.2 cm3 minimum in RPMI.
  • Fluids and FNAs: Equal parts RPMI and specimen.
Storage and Transportation

Specimens should be received at NeoGenomics within 72 hours from collection to assure sample integrity and acceptable cell viability. Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

CPT Code(s)*
88184(x1), 88185(x23), 88189(x1).
Turnaround Time

1 day

Level of Service
Global

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.