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Alternative Name
Ubiquitin specific peptidase 6
Test Description

Probe(s): USP6 (17p13.2)
Disease(s): Nodular fasciitis, aneurysmal bone cysts

Clinical Significance

USP6 gene rearrangements (fusions) are commonly found in nodular fasciitis (NF), aneurysmal bone cysts (ABC), myositis ossificans (MO), fibro-osseous pseudotumor of digit (FOTD), and fibroma of tendon sheath. Presence of a USP6 rearrangement can serve as a valuable marker for differentiating these neoplasms, many of which are self-limiting, from other neoplasms and malignancies with similar histological appearance.

Specimen Requirements
  • Bone marrow aspirate: N/A
  • Peripheral blood: N/A
  • Fresh, unfixed tissue: N/A.
  • Fluids: N/A
  • Paraffin block: Send paraffin block. Also send circled H&E slide for tech-only (required).
  • Cut slides: H&E slide (required) plus 4 unstained slides cut at 4-5 microns. Circle H&E slide for tech-only. 
  • Reminder about decalcified specimens: Testing will be attempted but most decalcification solutions are incompatible with FISH. Limited acid decalcification (<24 hours) in 5% formic acid or EDTA decalcification may better preserve DNA for FISH.
Storage and Transportation

Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

CPT Code(s)*
88374x1 automated or 88377x1 manual
New York Approved
Level of Service
Turnaround Time

5 days 3 for tech-only

  1. Hiemcke-Jiwa L, van Gorp JM, Fisher C, et al. USP6-associated neoplasms: A rapidly expanding family of lesions. Int J Surg Path. 2020;28(8):816-825.

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.