The Universal Solid Tumor NGS Fusion Panel is an RNA-based next-generation sequencing panel that detects translocations and fusions with known and novel fusion partners of these genes: ABL1, ACSL3, ACTB, ACTL6A, AFDN, AFF1, AFF3, AFF4, AHRR, AKAP9, AKT3, ALK, AR, ARID1A, ASPSCR1, ATF1, ATIC, AXL, BCOR, BCR, BRAF, BRCA1, BRCA2, BRD4, C11orf95, CAMTA1, CANT1, CAPZA2, CARS1, CBFA2T3, CCDC170, CCDC6, CCNB3, CCND1, CCND2, CCND3, CD274, CD74, CDH11, CDK4, CDK6, CDKN2D, CHCHD7, CIC, CIITA, CLTC, CNBP, COA5, COL1A1, COL1A2, CREB1, CREB3L1, CREB3L2, CREBBP, CRTC1, CRTC3, CSF1, CTLA4, CTNNB1, CTNNBL1, DDIT3, DDX3X, DDX5, DHH, DNAJB1, DUX4, EGFR, EGFRvIII, ELK4, ELL, EML4, EPC1, EPS15, ERBB2, ERG, ESR1, ESRP1, ETV1, ETV4, ETV5, ETV6, EWSR1, EZR, FAM131B, FEV, FGFR1, FGFR2, FGFR3, FGFR4, FLI1, FLT3, FOXO1, FOXP1, FRK, FUS, GLI1, GLIS1, GLIS2, GLIS3, GNAS, GOPC, HAS2, HERPUD1, HEY1, HIP1, HMGA2, HMGN2P46, HNRNPA2B1, IL2RB, IRF4, ITK, JAK2, JAZF1, KAT6A, KDM5A, KIAA1549, KIF5B, KIT, KLK2, KMT2A, KNL1, KRAS, LIFR, LMNA, LPP, MAML1, MAML2, MAST1, MAST2, MEAF6, MET, MET Exon 14 skipping, MKRN1, MLLT1, MLLT10, MLLT11, MLLT3, MN1, MPRIP, MRTFB, MSH2, MYB, MYBL1, MYC, MYH9, MYLK, NAB2, NCOA1, NCOA2, NCOA4, NDRG1, NFATC2, NFIB, NOTCH1, NOTCH2, NPM1, NR4A3, NRG1, NTRK1, NTRK2, NTRK3, NUP214, NUP98, NUTM1, NUTM2A, NUTM2B, OMD, PAN3, PATZ1, PAX3, PAX7, PAX8, PBX1, PCM1, PDGFB, PDGFRA, PDGFRB, PHF1, PIK3CA, PLAG1, PML, POU5F1, PPARG, PRCC, PRKACA, PRKAR1A, PRKD1, PRKD2, PRKD3, PTPRK, RAD51B, RAF1, RANBP2, RARA, RASGEF1A, RET, RHEBL1, ROS1, RPS6KC1, RSPO3, RUNX1, RUNX1T1, SDC4, SEC31A, SEPTIN6, SEPTIN9, SET, SLC34A2, SLC45A3, SND1, SNURF, SRF, SRGAP3, SS18, SSX1, SSX2, SSX4B, STAT6, STIL, STRN, SUZ12, SYK, TACC3, TAF15, TAL1, TBL1XR1, TCF12, TCF3, TCF7L2, TEAD1, TEAD2, TEAD3, TFE3, TFG, THADA, THRAP3, TMPRSS2, TP63, TPM3, TPM4, TPR, TRIM24, UBTF, USP6, VTI1A, WDFY2, WIF1, WT1, WWTR1, YAP1, YWHAE, and ZNF444 (total 250 genes and 2 variants).
Genomic rearrangements called gene fusions are present in approximately 20-30% of all cancers. Identification of translocations can be useful for diagnosis, disease sub-classification, and therapy determination.
The Universal Solid Tumor NGS Fusion Panel detects gene fusions across multiple solid tumors, including lung, brain, breast, thyroid, salivary gland, prostate, sarcoma, colorectal, cholangiocarcinoma, and pancreas. Compared to FISH, molecular detection of gene fusions, as provided in this test, requires less tumor sample for a much broader and therefore more cost-effective screen.
This test should be considered for difficult-to-diagnose tumors of uncertain histogenesis, particularly in younger patients (<50 years of age).
- FFPE tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.