Topoisomerase I (TOPO1) is essential nuclear enzyme for replication and transcription during cell reproduction and DNA repair. Upregulation of TOPO1 increases with disease stage in colorectal and pancreatic cancers.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.
Global: 48 hours, Tech-Only (stain only): 24 hours