Bi-directional sequencing of the entire coding sequence of the TET2 transcript variant A (2002 amino acids in length).
TET2 functions in DNA methylation as a part of epigenetic regulation of gene expression. Mutations are detected in myeloid cancers including 19% of MDS, 12% of MPN, 16% of AML, and 22% of CMML, and are associated with poor overall survival in intermediate-risk AML. Mutations are also detected in 38% of peripheral T-cell lymphoma and 47% of angioimmunoblastic T-cell lymphoma. In these lymphomas, TET2 mutations are associated with aggressive disease and poor outcome.
Peripheral blood: 5 mL in EDTA tube. Bone marrow: 2 mL in EDTA tube.
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. Ship same day as drawn whenever possible; specimens <72 hours old preferred.