Alternative Name
T-cell leukemia 1
Methodology
FISH
Test Description
Probes: TCL1 (14q32.1)
Disease(s): mature T-cell leukemia, T-cell prolymphocytic leukemia (T-PLL), adult T-cell leukemia/lymphoma (ATLL)
Clinical Significance
This breakapart probe covers breakpoint regions in the TCL1 (T-cell leukemia) gene cluster on chromosome 14q32 which encompasses TCL1A, TCL1B, and other genes. TCL1 translocations and inversions occur in mature T-cell leukemias including T-cell prolymphocytic leukemia (T-PLL) and adult T-cell leukemia/lymphoma (ATLL). These rearrangements cause gene overexpression due to juxtaposition with TCR-alpha or TCR-beta regulatory elements and contribute to oncogenesis.
Specimen Requirements
Bone Marrow Aspirate: 1-2 mL sodium heparin tube. EDTA tube is acceptable. Peripheral Blood: 2-5 mL sodium heparin tube. EDTA tube is acceptable. Fresh, Unfixed Tissue: Tissue in RPMI. Fluids: Equal parts RPMI to specimen volume. Paraffin block:  Send paraffin block. Also send circled H&E slide for tech-only (required). Cut slides: H&E slide (required) plus 4 unstained slides cut at 4-5 microns. Circle H&E slide for tech-only.
Storage and Transportation
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88377x1 manual or 88374x1 automated.
Turnaround Time
4 days for unfixed or FFPE specimens
Level of Service
Global
Technical

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.