Probes: TCL1 (14q32.1)
Disease(s): mature T-cell leukemia, T-cell prolymphocytic leukemia (T-PLL), adult T-cell leukemia/lymphoma (ATLL)
This breakapart probe covers breakpoint regions in the TCL1 (T-cell leukemia) gene cluster on chromosome 14q32 which encompasses TCL1A, TCL1B, and other genes. TCL1 translocations and inversions occur in mature T-cell leukemias including T-cell prolymphocytic leukemia (T-PLL) and adult T-cell leukemia/lymphoma (ATLL). These rearrangements cause gene overexpression due to juxtaposition with TCR-alpha or TCR-beta regulatory elements and contribute to oncogenesis.
- Bone Marrow Aspirate: 1-2 mL sodium heparin tube. EDTA tube is acceptable.
- Peripheral Blood: 2-5 mL sodium heparin tube. EDTA tube is acceptable
- Fresh, Unfixed Tissue: Tissue in RPMI
- Fluids: Equal parts RPMI to specimen volume
- Paraffin block: Send paraffin block. Also send circled H&E slide for tech-only (required). *** Testing on this specimen type is not yet available for NY specimens.***
- Cut slides: H&E slide (required) plus 4 unstained slides cut at 4-5 microns. Circle H&E slide for tech-only.
- Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen. For fresh samples: ship same day as drawn whenever possible; specimens <72 hours old preferred.
3-5 days for unfixed or FFPE specimens