T-cell leukemia 1
Probes: TCL1 (14q32.1)
Disease(s): mature T-cell leukemia, T-cell prolymphocytic leukemia (T-PLL), adult T-cell leukemia/lymphoma (ATLL)
This breakapart probe covers breakpoint regions in the TCL1 (T-cell leukemia) gene cluster on chromosome 14q32 which encompasses TCL1A, TCL1B, and other genes. TCL1 translocations and inversions occur in mature T-cell leukemias including T-cell prolymphocytic leukemia (T-PLL) and adult T-cell leukemia/lymphoma (ATLL). These rearrangements cause gene overexpression due to juxtaposition with TCR-alpha or TCR-beta regulatory elements and contribute to oncogenesis.
Bone Marrow Aspirate: 1-2 mL sodium heparin tube. EDTA tube is acceptable.
Peripheral Blood: 2-5 mL sodium heparin tube. EDTA tube is acceptable
Fresh, Unfixed Tissue: Tissue in RPMI
Fluids: Equal parts RPMI to specimen volume
Paraffin block: Send paraffin block. Also send circled H&E slide for tech-only (required)
Cut slides: H&E slide (required) plus 4 unstained slides cut at 4-5 microns. Circle H&E slide for tech-only.
Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Storage and Transportation
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
88377x1 manual or 88374x1 automated.
4 days for unfixed or FFPE specimens