This test provides qualitative detection of monoclonal T-cell receptor (TCR) beta gene rearrangements by PCR and fragment analysis according to BIOMED-2 consensus primer design. This test may be ordered concurrently with or after negative results in our T-Cell Receptor Gamma Gene Rearrangement assay for gamma gene rearrangements to improve TCR rearrangement detection by ~5% in T-cell leukemias/lymphomas.
T-cell receptor (TCR) gene rearrangement analysis is commonly used for determining clonality in the diagnostic evaluation of T-cell lymphomas and leukemias. TCR gamma gene (tested separately) and beta gene rearrangement analysis (as provided in this test) together will detect most clonal TCR rearrangements in patients with T-cell lymphomas/leukemias. Results should be interpreted in clinical context for diagnosis of T-cell lymphoproliferative disorders.
Peripheral blood: 5 mL in EDTA tube.
Bone marrow: 2 mL in EDTA tube.
FFPE tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Fresh tissue: Two pieces minimum, 0.2 cm^3 in RPMI.
Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Refrigerate fresh tissue until shipping. For all specimens, use cold pack for transport. Make sure cold pack is not in direct contact with specimen.