This test provides qualitative detection of monoclonal T-cell receptor (TCR) beta gene rearrangements by PCR and fragment analysis according to BIOMED-2 consensus primer design. This test may be ordered concurrently with or after negative results in our T-Cell Receptor Gamma Gene Rearrangement assay for gamma gene rearrangements to improve TCR rearrangement detection by ~5% in T-cell leukemias/lymphomas.
T-cell receptor (TCR) gene rearrangement analysis is commonly used for determining clonality in the diagnostic evaluation of T-cell lymphomas and leukemias. TCR gamma gene (tested separately) and beta gene rearrangement analysis (as provided in this test) together will detect most clonal TCR rearrangements in patients with T-cell lymphomas/leukemias. Results should be interpreted in clinical context for diagnosis of T-cell lymphoproliferative disorders.
- Peripheral blood: 5 mL in EDTA tube.
- Bone marrow: 2 mL in EDTA tube.
- FFPE tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
- Fresh tissue: Two pieces minimum, 0.2 cm^3 in RPMI.
Refrigerate fresh tissue until shipping. For all specimens, use cold pack for transport. Make sure cold pack is not in direct contact with specimen.