Methodology
Immunohistochemistry (IHC)
Test Description
Spirochete (Treponema pallidum) is the causative agent of syphilis. In the past, localization of the spirochete agent was achieved with silver stains such as Steiners and/or Warthin-Starry. Treponema pallidum can now be successfully localized with IHC techniques in FFPE tissue. The antibody consists of a rabbit purified IgG fraction and is highly specific for spirochete. Treponema palladium also cross-reacts with Borrelia burgdorferi (Lyme disease).
Specimen Requirements
- A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
or - One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
- Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage & Transportation
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88342 x 1 or 88341 x 1
Turnaround Time
Global: 48 hours, Tech-Only (stain only): 24 hours
Level of Service
Global
Stain Only
New York Approved
Yes