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Alternative Name
RAS Mutation Analysis, RAF Mutation Analysis, RAS RAF, RAS RAF Panel
Methodology
Molecular
Test Description

The RAS/RAF Panel is an NGS-based assay performed by sequencing the entire coding region (full gene) of BRAF, HRAS, KRAS and NRAS genes. The panel reports mutations detected in the full gene including mutations in the most common hotspots, if present. The common hotspots include KRAS (exons 2-4, including codons 12, 13, 59, 61, 117, and 146), NRAS (exons 2-4, including codons 12, 13, 59, 61, 117, and 146), HRAS (exons 2-3, including codons 12, 13, 59, and 61), and BRAF (exons 11, 15 including codon V600).

Clinical Significance

KRAS, NRAS, HRAS, and BRAF are members of the RAS/RAF/MAPK pathway. Current guidelines recommend KRAS and NRAS testing in metastatic colorectal cancer for determination of anti-EGFR therapy, and recommend BRAF testing as a marker of poor prognosis. BRAF mutations may predict lack of response to anti-EGFR therapy; evidence is mixed.

Specimen Requirements
  • FFPE tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Storage and Transportation

Use cold pack for transporting block during summer to prevent block from melting.  Slides can be packed at room temperature.

CPT Code(s)*
81479x1
Turnaround Time

10-14 days

Level of Service
Global

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.