The RAS/RAF Panel is an NGS-based assay performed by sequencing the entire coding region (full gene) of BRAF, HRAS, KRAS and NRAS genes. The panel reports mutations detected in the full gene including mutations in the most common hotspots, if present. The common hotspots include KRAS (exons 2-4, including codons 12, 13, 59, 61, 117, and 146), NRAS (exons 2-4, including codons 12, 13, 59, 61, 117, and 146), HRAS (exons 2-3, including codons 12, 13, 59, and 61), and BRAF (exons 11, 15 including codon V600).
RAS/RAF Panel
- Next Generation Sequencing (NGS)
Specimen Requirements
FFPE tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Storage and Transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen. NYS clients please provide date and time of Collection.
CPT Code(s)*
81404 - HRAS, 81405 - KRAS, 81406 - BRAF, 81479 - NRAS
Turnaround time
14 Days
Level of Service
- Global
Integrations
Request electronic integrations →
New York Approved: Yes
*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.
Biomarkers
RNA Sequencing
| Fusions | |||||
|---|---|---|---|---|---|
| BRAF | HRAS | KRAS | NRAS | ||
Last Updated: March 02, 2026