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Alternative Name
Rapid AML Panel
Methodology
Molecular
Test Description

The Rapid AML Therapeutic Panel analyzes 13 biomarkers through a combination of bi-directional Sanger sequencing, fragment analysis, and FISH as listed below.

  • Sanger sequencing (6 genes): FLT3 (ITD and TKD), NPM1, CEBPA, IDH1/IDH2, and TP53.
  • FISH probes: 5q-, -5 (5p15, 5q31, 5q33) | 7q-, -7 (Cen 7, 7q22, 7q31) | RUNX1/RUNX1T1 (ETO/AML1) t(8;21) | MLL (11q23) | PML/RARA t(15;17) | CBFB inv(16), t(16;16) | 17p- (TP53 17p13.1, NF1 17q11.2)

Test reports include a summary of all results together.

Clinical Significance

The Rapid AML Therapeutic Panel identifies genetic abnormalities associated with Acute Myeloid Leukemia (AML) that are useful for risk stratification and therapeutic decision making. This panel utilizes a combination of bi-directional Sanger sequencing, fragment analysis and FISH with a fast turnaround time. AML is usually an in-patient hematologic diagnosis and prompt time to treatment assignment can improve patient outcomes significantly.

Specimen Requirements
  • Bone Marrow Aspirate: 2-3 mL sodium heparin tube. EDTA tube is acceptable.
  • Peripheral Blood: 3-5 mL sodium heparin tube. EDTA tube is acceptable.
  • Fluids: Equal parts RPMI to specimen volume.
  • Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Storage and Transportation

Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.

CPT Code(s)*
Molecular: Client-bill only, if ordered concurrently with NeoTYPE® Myeloid Disorders Profile, AML Prognostic Profile, or MDS/CMML Profile. If ordered alone: 81245x1, 81246 x1, 81310 x1, 81218 x1, 81120 x1, 81121 x1, 81405 x1. FISH: 88374x7
Turnaround Time

4-5 Days for FLT3, IDH1/IDH2, and FISH. 7-10 Days for NPM1, CEBPA, and TP53.

Level of Service
Global

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.