Bi-directional sequencing to detect mutations in exons 19, 20, and 24, covering amino acid ranges W646 to S679, A681 to M743, and I839 to V860. Testing is available separately or in combination with the BTK Inhibitor Acquired Resistance Panel. NeoGenomics recommends ordering the combination Panel.
PLC-gamma-2 is immediately downstream of BTK in B-cell regulation pathways. Mutations in PLCG2 are associated with acquired or secondary resistance to ibrutinib arising after initial response in CLL.
Peripheral blood: 5 mL in EDTA tube. Bone marrow: 2 mL in EDTA tube.
FFPE tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns.
Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.