Alternative Name
PDGFR Alpha Rearrangement
Methodology
FISH
Test Description
Probes: PDGFRA | CHIC2 | FIP1L1 (4q12)
Disease(s): Chronic eosinophilic leukemia, MPN
Clinical Significance
FISH for FIP1L1-PDGFRA is generally found in chronic eosinophilic leukemia, but the presentation can be as acute myeloid leukemia, T-lymphoblastic lymphoma or both simultaneously. Detection of this abnormality predicts responsiveness to tyrosine kinase inhibitors. PDGFRA rearrangement is usually cryptic by routine cytogenetic analysis.
Specimen Requirements
- Bone Marrow Aspirate: 1-2mL Sodium Heparin Tube. EDTA tube is acceptable
- Peripheral Blood: 2-5mL Sodium Heparin Tube. EDTA tube is acceptable
- Fresh, unfixed tissue: Tissue in RPMI
- Fluids: Equal parts RPMI to specimen volume.
- Paraffin block: Send paraffin block. Also send circled H&E slide for tech-only (required).
- Cut slides: H&E slide (required) plus 4 unstained slides cut at 4-5 microns. Circle H&E slide for tech-only.
- Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
Storage & Transportation
Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: ship same day as drawn whenever possible; specimens <72 hours old preferred.
CPT Code(s)*
88374x1 automated or 88377x1 manual.
Turnaround Time
3-5 days
Level of Service
Technical
Global
New York Approved
Yes