Alternative Name
PDGFR Alpha Amplification
Methodology
FISH
Test Description

Probes: PDGFRA (4q12) | Centromere 4
Disease(s): Brain cancer

Clinical Significance

Amplifications of PDGFRA as detected by FISH occur in approximately 30% of pediatric and 20% of adult high-grade astrocytomas. In de novo adult glioblastoma multiforme (GBM), the co-occurrence of PDGFRA amplification with IDH1 mutation is associated with significantly worse overall survival compared to patients negative for amplification. Clinical trials with tyrosine kinase inhibitors targeting activated or over-expressed PDGFRA are in progress.

Specimen Requirements

Bone marrow aspirate: N/A Peripheral blood: N/A Fresh, unfixed tissue: N/A Fluids: N/A Paraffin block: Send paraffin block. Also send circled H&E slide for tech-only (required). Cut slides: H&E slide (required) plus 4 unstained slides cut at 4-5 microns. Circle H&E slide for tech-only.

Storage and Transportation

Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.

CPT Code(s)*
88377x1 manual or 88374x1 automated.
Turnaround Time

3-5 days

Level of Service
Global
Technical

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.