Immunohistochemistry (IHC)
Test Description
The Pan Melanoma staining combination increases sensitivity for melanomas. Since many skin biopsies are small, the triple stain conserves valuable tissue while saving time. Melan A (brown cytoplasmic) is a useful addition to melanoma panels as it is specific for melanocytic lesions. Tyrosinase (brown cytoplasmic) is a key enzyme involved in the initial stages of melanin biosynthesis. Tyrosinase is a more sensitive marker when compared to HMB45 and MART-1. It also labels a higher percentage of desmoplastic melanomas than HMB45. S100 stains Schwannomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metastases. S100 (nuclear red) protein is also expressed in antigen presenting cells, such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.
Specimen Requirements
  • A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type


  • One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered

  • Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.

Storage and Transportation
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88344 x 1
Turnaround Time
24 hours
Level of Service
Stain Only

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.