Methodology
Immunohistochemistry (IHC)
Test Description
Octamer Binding Transcription Factor 4 (OCT4, also known as OCT3/4, POU51) is a transcription factor and is expressed by early embryonic cells, germ cells, and stem cells. OCT4 is a nuclear marker of classical seminoma and embryonal carcinoma. It has excellent sensitivity and specificity for these two tumors, and can be used as the "screen" for these neoplasms when dealing with a metastatic tumor of unknown origin.
Specimen Requirements
- A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
or - One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
- Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage & Transportation
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88342 x 1 or 88341 x 1
Turnaround Time
24 hours
Level of Service
Stain Only
New York Approved
Yes