Alternative Name
Nuclear protein in testis
Methodology
Immunohistochemistry (IHC)
Test Description

Expression of nuclear protein in testis (NUT) is generally confined to the germ cells of the testis and ovary. NUT midline carcinomas are aggressive tumors with non-diagnostic morphology that overlaps with many other poorly differentiated tumors. In these tumors, rearrangement of the NUT gene at chromosome 15q14 causes NUT protein overexpression. IHC staining with NUT antibody may serve as a diagnostic alternative to FISH or molecular confirmation of 15q14 rearrangement. 

Specimen Requirements
  • A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
    or
  • One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
  • Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage and Transportation

Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.

CPT Code(s)*
88342x1 or 88341x1
Turnaround Time

24 hours

References
Park HS, Bae YS, Yoon SO et al. Usefulness of NUT IHC in the cytodiagnosis of NUT midline carcinoma: a brief case report.Korean J Pathol.2014;48:335-338.
Level of Service
Stain Only

*The CPT codes provided with our test descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.