Expression of nuclear protein in testis (NUT) is generally confined to the germ cells of the testis and ovary. NUT midline carcinomas are aggressive tumors with non-diagnostic morphology that overlaps with many other poorly differentiated tumors, but are characterized by rearrangement of the NUTM1 (NUT) gene at chromosome 15q14 with BRD4/3 (and rarely NSD3, ZNF532 or ZNF592), which causes NUT protein overexpression. IHC staining with NUT antibody may serve as a diagnostic alternative to FISH or molecular confirmation of 15q14 rearrangement. However, NUT overexpression by IHC may also be seen in other diseases NUTM1 rearrangement, such as porocarcinoma with YAP1-NUTM1 gene fusion (PMID: 32436169), so a diagnosis of NUT midline carcinoma should not be based on NUT IHC positivity alone.
- A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
or - One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
- Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
24 hours
- Agaimy A, Tögel L, Haller F, Zenk J, Hornung J, Märkl B. YAP1-NUTM1 Gene Fusion in Porocarcinoma of the External Auditory Canal. Head Neck Pathol. 2020;14(4):982-990.
- Napolitano M, Venturelli M, Molinaro E, Toss A. NUT midline carcinoma of the head and neck: current perspectives. Onco Targets Ther. 2019;12:3235-3244.
- Park HS, Bae YS, Yoon SO, et al. Usefulness of Nuclear Protein in Testis (NUT) Immunohistochemistry in the Cytodiagnosis of NUT Midline Carcinoma: A Brief Case Report. Korean J Pathol. 2014;48(4):335-338.