Methodology
FISH
Test Description
Probes: NTRK3 (15q25.3)
Disease(s): Various solid tumor cancers with high incidence in IFS, MASC, SBC, CMN
Clinical Significance
NTRK3 FISH is useful in confirming diagnoses and/or screening for TRK inhibitor options in infantile (congenital) fibrosarcoma (IFS), mammary analogue secretory carcinoma (MASC), secretory breast carcinoma (SBC), and congenital mesoblastic nephroma (CMN). FISH panel testing for NTRK1, 2, and 3 rearrangements (coming soon) is recommended for pediatric glioma, papillary thyroid carcinoma (PTC) and other tumors. The NTRK3 gene encodes Trk-C protein.
Specimen Requirements
- Bone marrow aspirate: N/A
- Peripheral blood: N/A
- Fresh, unfixed tissue: N/A.
- Fluids: N/A
- Paraffin block: Send paraffin block. Also send circled H&E slide for tech-only (required).
- Cut slides: H&E slide (required) plus 4 unstained slides cut at 4-5 microns. Circle H&E slide for tech-only.
Storage & Transportation
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.
CPT Code(s)*
88377x1 manual or 88374x1 automated
Turnaround Time
3-5 days
Level of Service
Global
Technical
New York Approved
Yes