In normal tissue, most neurons and their axonal and dendritic processes stain strongly positive for Neuron Specific Enolase (NSE), with the exception of Purkinje cells. Schwann cells, cells of the adrenal medulla, and paraganglia also contain NSE. Endocrine cells of the skin (Merkel cells), respiratory and GI tract epithelium, pituitary parathyroid, and pancreatic islets and C cells of thyroid all stain positively for NSE. NSE is expressed in ganglioneuromas, neuroblastomas, Schwannomas and malignant melanomas. It is also present in pheochromocytomas and paragangliomas. Carcinoids, medullary thyroid carcinomas, pituitary adenomas and endocrine tumors of the pancreas and GI tract all show positive immunoreactivity for NSE. NSE is found in neuroendocrine carcinoma of the skin (Merkel cell tumor) and small cell carcinoma of the lung.
- A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type
or - One (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody ordered
- Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.
24 hours
Global prognostic interpretation is available (TAT is 48 hours)