The NGS Rhabdomyosarcoma Fusion Profile is a targeted next-generation sequencing panel that can detect various translocations related to rhabdomyosarcoma in the genes FOXO1, NCOA2, and TFE3.
The diagnosis and subclassification of rhabdomyosarcoma is very important for diagnostic, prognostic and treatment purposes. Several genetic abnormalities are associated with specific subtypes of rhabdomyosarcoma. In addition, alveolar soft part sarcoma is a soft tissue sarcoma that may be easily confused with certain types of rhabdomyosarcoma. This fusion profile is designed to aid in the diagnosis and subclassification of rhabdomyosarcoma and to identify cases of alveolar soft part sarcoma.
FFPE tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Use cold pack for transporting block during summer to prevent block from melting. Slides can be packed at room temperature.